Endothelial MerTK impairment promotes atherosclerotic plaque formation. To explore the role of endothelial MerTK in atherosclerosis and validate the signaling pathways from big data analytics and human microarray analysis, we established an early stage of atherosclerosis model in MerTKflox/flox mice and MerTKflox/floxTie2Cre mice. This evidence concerns the gene MERTK and atherosclerosis.