Downstream analyses reveal spatially variable genes (e.g., TMSB10 in cortical layers of the DLPFC dataset,[21] and ERBB2 in tumor regions of HER2+ breast cancer dataset[22]), and Gene Ontology (GO) enrichment uncovers tissue‐specific biological processes (e.g., immune response within the tumor microenvironment). This evidence concerns the gene ERBB2 and breast cancer.