Taken together, we speculated that the functions of EMT, bile acid metabolism, and oxidative phosphorylation; as well as the signaling pathways of IL-2-STAT5, VEGF, PPAR, mTORC1, and TNF-α signaling via NF-κB, enriched by the identified 144 DEPs (such as XPOT, RFC4, EDN2, SF3B4, and PRPSAP2, RDH11, CPSF2, IGHA2, CPM, and COQ9), may have crucial effects on PCOS evolvement. Here, CPSF2 is linked to polycystic ovary syndrome.