To test if ACTL6A is correlated with p-ERK expression in human HNSCC tissue, we performed immunofluorescence staining for both proteins on freshly cut tissue microarray (TMA) of ten HNSCC tumors and two normal upper aerodigestive tract mucosa specimens (Fig. 6D). The gene discussed is ACTL6A; the disease is head and neck squamous cell carcinoma.