Infection of BMDMs with M. kansasii, the most pathogenic nontuberculous mycobacterium(28, 29), also resulted in robust necroptosis dependent on Mlkl in the presence of z-VAD but not Q-VD-OPH, while M. abscessus or M. marinum combined with z-VAD were not able to induce necroptosis (Figure 4A). This evidence concerns the gene MLKL and infection.