Given that Mlkl is an ISG and previous reports showed that Sp140−/− mice had elevated IFN-I levels after Mtb infection due to abrogation of negative regulation of Ifnb1 mRNA by SP140 (32, 33), we wondered whether the deficiency of SP140 would potentiate the induction of Mlkl and affect TB disease progression in mice. Here, MLKL is linked to tuberculosis.