In vitro: Brachydin B demonstratedselective cytotoxicity toward DU145 cancer cells without affectingnontumoral HGF cells at low doses. In 2D culture, cytotoxicity wasconfirmed via MTT, LDH release, and triple staining,which showed no signs of apoptosis/necrosis at lower concentrations.In 3D spheroids, brachydin B inhibited viability and volume growthat high concentrations and long exposure times. No in vivo toxicity assessment was performed. The gene discussed is HGF; the disease is cancer.