In vitro: Cytotoxicity was evaluatedusing multiple assays: MTT assay (570 nm) and Neutral Red uptake assessed cell viability; LDH release assay (340 nm) measured membrane damage; AO/EB fluorescencemicroscopy (515–560 nm) andAnnexin V/PI flow cytometry distinguished apoptotic and necrotic cells.The extract showed selective cytotoxicity toward tumor cells, withnecrosis at 30–60 mg/mL, increasedNBUDs, and downregulation of BCL-XL, BIRC5, and MET, suggesting genomicinstability and impaired cell survival mechanisms. This evidence concerns the gene BIRC5 and neoplasm.