AKT1 and cancer: Focus on the antioxidant assay and antitumor activity. Invitro: All compounds showed cytotoxicity against PC-3prostate cancer cells. Brachydin B and brachydin C, (0.96–6 μM) for 1–24 h, induced both apoptosis and necrosis, while brachydinA (6 μM, 24 h) predominantly induced necrosis at higher concentrations. Elevatedcleaved PARP expression supported apoptosis. Brachydin B and brachydinC upregulated p21, suggesting G1 arrest; brachydin A and brachydinB decreased phospho-AKT levels. No genotoxicity was observed in thecomet assay