By analyzing the LR pairs of communication between MF2 and hepatocytes in HCC patients with MVI absent and MVI present, it was found that direct contact LR pairs, including OCLN-OCLN, JAG1-NOTCH3, CDH5-CDH5, CD99−CD99, and APP-CD74, were involved in mediating the communication between MF2 cells and hepatocytes in both HCC patients with MVI absent and MVI present. The gene discussed is NOTCH3; the disease is hepatocellular carcinoma.