In an attempt to identify the target through which AGO2 suppresses interferon responses in the virus infection model, we evaluated IFNβ promoter-driven luciferase activities induced by RLR signaling ligands, including 5′ppp-IVT RNA, viral RNA, 5′ppp-dsRNA, or downstream effectors in the RIG-I pathway, including 2CARD, TBK1, IRF3, and IRF3-5D, using AGO2 knockout 293 cells (Figure 4B). Here, IRF3 is linked to viral infectious disease.