Since FHL2 localizes at focal adhesions (FAs) and stress fibers (SFs) in the cell on rigid surfaces, we first checked the localization of FHL2-GFP-BirA in U2OS cells, a human osteosarcoma cell line, which were cultured on the glass surface (Nakazawa et al. 2016). This evidence concerns the gene FHL2 and osteosarcoma.