Furthermore, we treated Apoe−/− mice with Ang II and conducted RNA-seq, and the results demonstrated TGF-β signaling pathway down-regulation in the aorta of AAA mice compared with Apoe−/− mice treated with saline, with TGFβ2 and TGFβ3 being the main down-regulated genes (Fig. 5B and C). The gene discussed is APOE; the disease is triple-A syndrome.