Analyzing IP-PSD-95 fragments and remnants, we observed a significant increase in ES-GluN2B subunit expression levels across all brain regions with AD progression, with the most marked increase in cortex and midbrain (ctx, Student’s two-tailed t-test, t (4) = 5.42, p = 0.005; mb, Student’s two-tailed t-test, t (4) = 14.7, p < 0.001, n ≥ 4; Figures 3A,B), with no observed changes in the cerebellum (Student’s two-tailed t-test, t(4) = 0.482, p = 0.650, n ≥ 4; Figure 3B). Here, DLG4 is linked to Alzheimer disease.