By comparing the differential methylation between cancerous and non-cancerous samples in the UALCAN database (Supplementary Figure S4A), we observed a correlation between higher MDH1 expression levels in LUAD, BLCA, thyroid carcinoma (THCA), and testicular germ cell tumors (TGCT) and reduced methylation levels (Supplementary Figure S4A). This evidence concerns the gene MDH1 and bladder transitional cell carcinoma.