Given the multifaceted role of EVs in MM biology, this study aimed to: i) characterize EVs isolated from BM plasma of MM patients; ii) assess the expression of the immune checkpoint molecules HLA-G, PD-1, and PD-L1 on these EVs; iii) investigate their immunoregulatory functions, with a particular focus on the roles of HLA-G and the PD-1/PD-L1 axis. This evidence concerns the gene PDCD1 and Miyoshi myopathy.