To delineate the cell type–specific transcriptional landscape of EGFR/TP53 co-mutated non–small cell lung cancer (NSCLC) and its tumor microenvironment (TME), we performed single-cell transcriptome sequencing on tumor specimens from 4 patients harboring concomitant EGFR and TP53 mutations using the 10x Genomics Chromium Fixed RNA Profiling platform. Here, EGFR is linked to lung cancer.