By intersecting DEGs with previously reported NETs-related genes, we identified five key genes: SRC, TLR8, FCAR, MAPK1 and HIF1A. Functional enrichment analysis revealed that these genes are significantly involved in immune regulatory pathways and leukocyte apoptosis, reinforcing their connection to NETs-mediated immune dysregulation in stroke. The gene discussed is MAPK1; the disease is stroke disorder.