Disease-associated astrocytes (DAA) have been identified as a sub-population of reactive astrocytes found in aged WT and 3xTg-AD animals by Habib et al.13The expression of these DAA genes, such as Gfap, Serpina3n, Vim, and Osmr are expressed by a relatively small number of the young, WT astrocytes included in the single-cell sequencing data analyzed here; however, interestingly, the expression of these genes is reduced over time after exposure to FUS-BBBO relative to naive controls (Figure 7A). This evidence concerns the gene OSMR and Alzheimer disease.