LY86 and Anxiety: This acute reaction was followed by an enduring pathological state that exhibited three hallmarks of severe chronic pain in humans26: bilateral hypersensitivity to mechanical and thermal stimuli (contralateral: Figures 5C and 5D; ipsilateral; Figure S10B),37,38 heightened anxiety-like behavior39 (Figures 5E and S10C), and long-term memory deficits (Figure 5F).40 MD-1 reduced the second, but not the first, phase of the formalin response (Figure 5B), attenuated edema (Figure S10A), and, most crucially, stopped all behavioral signs of chronic pain (Figures 5C–5F, S10B, and S10C).