Validation analysis adjusting the correlation for the influential factors, such as age and duration, of MS shows that the strongest mutual relationship is between EDSS and neurofilaments (0.21) in RR MS patients, and in SPMS patients, EDSS is most strongly correlated with SEMA3A (0.32) and with progranulin (0.21); see Supplementary Table S2. The gene discussed is GRN; the disease is secondary progressive multiple sclerosis.