To comprehensively define the molecular characteristics of MYCN-driven tumorigenesis in human retinal organoids, we performed transcriptomic profiling and compared MYCN-overexpressing retinoblastoma organoids (MYCNO/E-RBOs), MYCNO/E-RBOs derived cell lines (MYCNO/E-cells), normal retinal organoids (nROs), and the RB1-deficient retinoblastoma cell line Y79. The gene discussed is RB1; the disease is retinoblastoma.