To further validate our retinal organoid model as a representative platform for studying MYCN-driven retinoblastoma, we performed Gene Set Enrichment Analysis (GSEA) comparing MYCNO/E-RBOs with nROs and independently analyzed publicly available datasets from MYCN-amplified patient retinoblastomas relative to RB1-deficient tumors. This evidence concerns the gene RB1 and retinoblastoma.