As we did not follow up with the loss of B7H6 expression on melanoma cells isolated ex vivo from a relapsed NSG mouse treated with NKp30-CD28 CAR TCRKO T therapy, we can only speculate that the appearance of a B7H6 negative melanoma clone was possibly due to dysfunctional transcriptional/post-transcriptional control mechanism described or metalloproteinase-mediated shedding. The gene discussed is NCR3LG1; the disease is melanoma.