IL-4, IL-10, and TNF-α levels in NLF were significantly increased in the CRS mouse model (IL-4: 6.7 ± 1.4 pg/mL; IL-10: 11.6 ± 3.2 pg/mL, and TNF-α: 7.8 ± 3.1 pg/mL) compared with the negative control mice (0.5 ± 0.1 pg/mL, 6.6 ± 1.7 pg/mL, and 1.1 ± 0.4 pg/mL, respectively). This evidence concerns the gene IL4 and congenital rubella syndrome.