In addition, we analyzed (I) liver function via the following serum markers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT) and alkaline phosphatase (AP); (II) morphological and histological changes in the liver; (III) alpha-fetoprotein (AFP) levels in hepatocytes (as a marker of HCC); and (IV) changes in the morphology of hepatocytes cultured with Mf and Mf-M1. This evidence concerns the gene DHCR7-DT and hepatocellular carcinoma.