Through ssGSEA enrichment analysis and correlation analysis, we found that, among all the m6A modified regulatory factors, the expression of RBMX was negatively correlated with most immune cells in the osteosarcoma TME, which suggested that RBMX may be a negative regulatory factor in the osteosarcoma microenvironment (Figure 1A). This evidence concerns the gene RBMX and osteosarcoma.