In this study, CAFs and TAMs were annotated based on their enrichment in tumor samples, co-expression of genes frequently associated with tumor-promoting phenotypes (e.g., ACTA2, FAP, and COL11A1 for CAFs [40]; CD163, MSR1, and CD206 as typical M2 macrophage markers [41]), along with their absence in normal tissues. The gene discussed is ACTA2; the disease is neoplasm.