Given the well-established roles of copper metabolism (mediated by chaperones such as ATOX1) and Rho/ROCK signalling in cancer pathogenesis, coupled with emerging evidence underscored by lung cancer models showing anti-tumour effects upon their inhibition, we aimed to comprehensively evaluate the expression profile, prognostic significance, and therapeutic potential of ATOX1 and ROCK1 in the context of LUAD. Here, ATOX1 is linked to lung cancer.