The increased potency of CF10 relative to 5-FU and TFT is general—as evidenced by lower AUC values over multiple CRC cell lines included in the Broad Institute PRISM screen is maintained with folate restriction, and is associated with stronger TS inhibition and potent Top1cc formation, consistent with mechanistic similarities to Top1 poisons [48]. The gene discussed is TOP1; the disease is colorectal carcinoma.