FN-EDA activates the Toll-Like Receptor 4/Nuclear Factor kappa-light-chain-enhancer of activated B cells (TLR4/NF-κB) pathway in HCC cells, leading to the increased expression of Serine Hydroxymethyltransferase 1(SHMT1), a crucial enzyme in one-carbon metabolism, which helps cancer cells counter sorafenib-induced oxidative stress [159]. The gene discussed is TLR4; the disease is cancer.