This panel of CAPN1, CAPN2, and CAPNS1 KO and R cell lines was used to explore the role of calpain-1/2 isoforms in cancer cell migration and invasion in vitro, including velocity and directionality [31], and in vivo tumor growth and metastasis using an orthotopic mouse engraftment model (similarly to [32]) in Rag2-/- IL2Rγc-/- mice [33]. The gene discussed is CAPN2; the disease is cancer.