No association were detected between TIM-3 expression on CD4 or CD8 T-lymphocytes and either age, gender, hepatomegaly, splenomegaly, lymphadenopathy, CNS infiltration, Hb level, platelet count, IPT, BM cellularity, BM lymphocytes%, CD34%, CD34+/CD38-%, CD3+/CD4+, CD3+/CD8+, CD4:CD8 ratio, molecular genetics, cytogenetic risk groups or DNA index. The gene discussed is HAVCR2; the disease is Splenomegaly.