In the PCOS-induced model, letrozole combined with a high-fat diet resulted in significant metabolic and histological alterations, consistent with typical PCOS pathology, including increased body weight, dyslipidemia, elevated testosterone and LH levels, suppressed FSH, oxidative stress, and inflammation, alongside ovarian structural damage with follicular degeneration and stromal infiltration. This evidence concerns the gene BRD2 and metabolic syndrome.