Though a distinct TSC population was not noted at the transcriptome level, proteomic analysis revealed a significant increase in TSC markers such as ubiquitin-like interferon-stimulated gene 15 (ISG15), stearoyl-CoA desaturase (SCD) and sorting nexin-2 (SNX2) in bioprinted compared to Matrigel organoids (Fig. 3d). This evidence concerns the gene ISG15 and tuberous sclerosis.