In addition, we also explored the effects of C3AR1 and ITGB2 overexpression on non-diabetic kidney disease, and the results showed that overexpression of C3AR1 increased C3AR1 and ITGB2 expression, while overexpression of ITGB2 increased ITGB2 protein levels, but had no significant effect on C3AR1 expression (Supplementary Figure 2). The gene discussed is ITGB2; the disease is kidney disorder.