For histologic analysis, tumor specimens of H22 mouse model were fixed with 4% paraformaldehyde, dehydrated in ethanol, embedded with paraffin, and stained with hematoxylin and eosin (H&E) and stained with CD4 (SouthernBiotech, 1540–01), CD8 (Servicebio, GB13429), IFN-γ (BioLegend 606,853) and Foxp3 (BioLegend 126,406) antibodies, respectively. The gene discussed is CD4; the disease is neoplasm.