In depression, deficiency of Kir6.1 activates the NLRP3 inflammasome through excessive accumulation of mitochondrial ROS, triggering caspase-1-dependent cleavage of gasdermin D (GSDMD), leading to astrocyte pyroptosis and the release of the pro-inflammatory cytokine IL-1β (82). The gene discussed is GSDMD; the disease is major depressive disorder.