To further confirm whether DUSP6 mediates mitophagy in bladder cancer through the mTOR pathway, we pre-treated DUSP6 knockdown cells with medium containing 1.5 μM of mTOR agonist MHY1485 for 12 hours, and observed that the level of mitophagy was decreased (Figure 7E). The gene discussed is MTOR; the disease is urinary bladder cancer.