The results of the colony formation assay demonstrated that TSPO overexpression significantly inhibited the colony-forming ability in KYSE30 (Figure 8E), KYSE450 (Figure 8F), and EC1 (Figure 8G) cells, indicating its inhibitory effect on the proliferation of esophageal cancer cells (all P < 0.001). The gene discussed is TSPO; the disease is esophageal cancer.