Immunohistochemical staining of isolated glioma samples revealed that, compared with those in the NC group, EGR1 and HOXB9 protein levels in glioma samples from the OE miR‐192 group were obviously lower (U87 group: 0.35 ± 0.07 vs. 1.10 ± 0.14; U251 group: 0.40 ± 0.14 vs. 1.25 ± 0.07; *p < 0.05; Figure 6E,F). This evidence concerns the gene HOXB9 and central nervous system cancer.