After 2 days of trametinib treatment in A549 cells, the top 1000 upregulated genes were enriched in pathways such as pathways related to cancer, the PI3K-AKT signaling pathway, the mitogen-activated protein kinase (MAPK) signaling pathway, and the apoptosis pathway; moreover, the top 1000 downregulated genes were enriched in pathways such as metabolic pathways, complement and coagulation cascades, tyrosine metabolism, and the MAPK signaling pathway (Supplementary Fig. 1d). The gene discussed is WNK2; the disease is cancer.