To further validate RNA-seq data, we used the same AML samples and performed real time PCR (qPCR) for five AR target genes which are known to be upregulated by androgen signaling in prostate cells (KLK2, KLK3, FKBP5, TMPRSS2 and AR) [22]. The gene discussed is KLK3; the disease is acute myeloid leukemia.