To further validate RNA-seq data, we used the same AML samples and performed real time PCR (qPCR) for five AR target genes which are known to be upregulated by androgen signaling in prostate cells (KLK2, KLK3, FKBP5, TMPRSS2 and AR) [22]. This evidence concerns the gene TMPRSS2 and acute myeloid leukemia.