GAD2 and Anxiety: To evaluate the sufficiency of elevated Cckar expression in promoting CCS-induced anxiety-like and aggressive behaviors, Cckar was overexpressed in mPOAGad2 neurons through the injection of a Cre-dependent Cckar-3xFLAG-P2A-EGFP virus (ovCckar) into the mPOA of Gad2-Cre mice (Fig. 4A).