qPCR analysis revealed that Sall4 expression is significantly elevated in myrAkt–YAP1S127A-driven CCA (AY-CCA), myrAkt–NRASG12V-driven cHCC-CCA (ANRAS-cHCC-CCA), and KRASG12D–shp53-driven mHCC/CCA (KP53-mHCC/CCA) compared with the healthy control livers. The gene discussed is SALL4; the disease is cholangiocarcinoma.