To investigate AllChAT, we performed chromatin immunoprecipitation followed by sequencing (ChIP–seq) for H3K27me3 to identify closed chromatin regions, and H3K4me3 for open chromatin in tumor patient-derived cells (PDCs) from TRACERx tumors (CRUK0977 and CRUK0557), and a PDC from NAT (CRUK0667). This evidence concerns the gene BRD2 and neoplasm.