To explore the role of SNAT6 in the cerebellum, we performed IHC against SNAT6 and found that it was widely expressed throughout the mouse brain but enriched in the PC layer of the cerebellum (Supplementary Fig. 8a), which was consistent with the expression pattern revealed by in situ hybridization.41 To examine the potential role of PCs in mediating tremor, we specifically deleted Slc38a6 in the PCs of mice (Slc38a6PC-/-) (Supplementary Fig. 8b), which also exhibited tremors that worsened with age (Supplementary Video 7), similar to those in Slc38a6-/- mice. Here, SLC38A6 is linked to Tremor.