Taken together, our results reveal multiple interrelated mechanisms by which HCMV infection impairs TEAD1 activity (Figure 6D): (1) Exclusion of TEAD1 exon 6 (Figure 6C); (2) Reduction of TEAD1 gene and TEAD1 protein levels (Figures 5D and 6A); (3) Reduction of YAP1 and pYAP1 protein levels (Figure 6A); and (4) Extensive closing of human chromatin that is normally occupied by TEAD1 (Figure 4). This evidence concerns the gene YAP1 and cytomegalovirus infection.