By ELISA, the MDA-MB-468 tumors of the mice treated with Doxy@CaCO3-PEG and Tet-HER1-CAR-T cells exhibited significant secretion of IL-2, IFN-γ and TNF-α, all of which promoted the cytolysis of tumor cells, at both Days 2 and 4 (Fig. 4A–D). Here, TNF is linked to neoplasm.