In our workflow, a duplex immunofluorescence staining panel consisting of CD8 and T-cell immunoglobulin and mucin-domain containing-3 (TIM3) was used to assess CD8+ TIM3- T cells, CD8+ TIM3+ exhausted T cells,11 and TIM3+ (presumed) macrophages on a tumor microarray (TMA) of high-grade serous carcinomas (HGSCs) and low-grade endometrial carcinomas. Here, CD8A is linked to endometrial carcinoma.