The patients with low VAF values included a 75-year-old female diagnosed with soft tissue sarcoma (TP53 p.K132R, VAF = 0.224 [124/553]) and a 62-year-old male diagnosed with pancreatic cancer (TP53 p.H179Y, VAF = 0.255 [116/455]). Here, TP53 is linked to soft tissue sarcoma.