To further investigate tumour cell durotaxis in vivo, we performed two-photon imaging on precision-cut slices of subcutaneous tumours formed in syngeneic mice using KPC mice expressing the YFP lineage tag (KPCY) cells, derived from Pdx1‐Cre; LSL‐KrasG12D/+; LSL-Trp53L/+; Rosa26YFP/YFP mice39 (Fig. 6a). This evidence concerns the gene PDX1 and neoplasm.