SCN8A and epilepsy: To assess circuit-specific effects of gain-of-function p.SCN8A mutations in DEE-13, we performed extracellular local field potential (LFP) recordings and two-photon calcium imaging (2PCI) of Cx+GE assembloids derived from two DEE-13 patients with distinct SCN8A variants (p.R1872L in P1 and p.V1592L in P2).7 P1 exhibited severe, treatment-resistant epilepsy, while P2 had a milder clinical profile with well-controlled seizures.7 For comparison, we used a CRISPR-corrected isogenic control (iCtrl) for P1.