To further investigate the function of ZEB1 on TIME, we performed single-cell RNA-Seq (scRNA-Seq) using the tumor tissue collected from mice allografted with KPC-shV or KPC-shZEB1 cells treated with gemcitabine shown in Figure 1, C, H, and I. Compared with control, ZEB1 KD substantially increased the proportion of total T cells and CD8+ T cells (Figure 1J, Supplemental Figure 1Q, and Supplemental Figure 2, A and B). This evidence concerns the gene ZEB1 and neoplasm.